glynp r406 free base  (MedChemExpress)

Journal: iScience

Article Title: Heat-killed Mycobacterium tuberculosis induces trained immunity in vitro and in vivo administered systemically or intranasally

doi: 10.1016/j.isci.2024.108869

Figure Lengend Snippet: Heat-killed Mycobacterium tuberculosis -induced trained immunity relies on epigenetic reprogramming, hypoxia-inducible factor-1α, and Syk kinase (A, C, and F) Peripheral blood mononuclear cells (PBMCs) were incubated with the methyltransferase inhibitor 5′-deoxy-5’-(methylthio) adenosine (MTA) (A), the hypoxia-inducible factor (HIF)-1α inhibitor PX-478 (C), and the Syk inhibitor R406 (F) before training with heat-killed Mycobacterium tuberculosis (HK Mtb ) for 1 day. Cells were then washed and left to rest for 5 days. Remaining adherent monocytes were washed and rechallenged with lipopolysaccharide (LPS) or R848 for another day, and tumor necrosis factor (TNF)α in the supernatants was analyzed by enzyme-linked immunosorbent assay (ELISA). (B, D and E) PBMCs were trained with HK Mtb for 1 day. Cells were then washed and left to rest for 5 days (B) Chromatin immunoprecipitation (ChIP) was performed against trimethyl-Histone H3 (Lys4), analyzing the enrichment of this residue at the TNFA promoter by quantitative polymerase chain reaction (qPCR). (D) Total RNA was extracted and HIF1A expression was analyzed by qPCR. (E) Supernatants were collected, and lactate was analyzed by a colorimetric assay. Each dot represents an independent donor. (A–D, F) N = 2–3, n = 6–9. (E) N = 6, n = 14. (B, D) Data are represented as mean ± SEM. Paired Student’s t test comparing control (not trained) versus trained cells or exposed versus not exposed to the various inhibitors (A, C, F). (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001).

Article Snippet: R406 free base , MedChemExpress , Cat# HY-11108.

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Chromatin Immunoprecipitation, Residue, Real-time Polymerase Chain Reaction, Expressing, Colorimetric Assay, Control

Journal: iScience

Article Title: Heat-killed Mycobacterium tuberculosis induces trained immunity in vitro and in vivo administered systemically or intranasally

doi: 10.1016/j.isci.2024.108869

Figure Lengend Snippet:

Article Snippet: R406 free base , MedChemExpress , Cat# HY-11108.

Techniques: Recombinant, Saline, Enzyme-linked Immunosorbent Assay, Isolation, Reverse Transcription, SYBR Green Assay, Lactate Assay, Staining, Software